激活免疫球蛋白基因的人类淋巴体特异性转录因子是一种同源盒蛋白质
C Scheidereit1, J A Cromlish, T Gerster
1Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Nature
|December 8, 1988
概括
人类转录因子OTF-2,对淋巴细胞至关重要,与Drosophila蛋白具有DNA结合的相似性. 这表明这些关键的调节元素和蛋白质有着共同的进化历史.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 转录因子调节基因表达.
- 宿主蛋白质是关键的DNA结合蛋白质,参与发育.
- OTF-2是一种人类淋巴细胞特异性的转录因子.
研究的目的:
- 研究人类转录因子OTF-2的DNA结合特异性.
- 为了比较OTF-2的DNA结合特性与无脊椎动物的同类基因蛋白.
主要方法:
- 对OTF-2的同类基因区域的分析.
- 用DNA结合测试来确定目标DNA元素.
- 与已知的Drosophila同居原体蛋白结合部位进行比较分析.
主要成果:
- 人类的OTF-2蛋白质具有对DNA结合至关重要的同类基因.
- OTF-2 特别与 DNA 元素结合.
- 这些元素也被来自Drosophila的同类基因蛋白所识别.
结论:
- 哺乳动物和无脊椎动物的同类基因蛋白具有保留的DNA结合特异性.
- 这表明同类基因蛋白和它们的DNA识别序列在物种之间共同进化.
- OTF-2 作为理解保留监管机制的模型.
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