一种Ultrabithorax蛋白与自身和Antennapedia P1促进体附近的序列结合
P A Beachy1, M A Krasnow, E R Gavis
1Department of Biochemistry, Stanford University School of Medicine, California 94305.
Cell
|December 23, 1988
概括
对于果发育至关重要的Ultrabithorax (Ubx) 蛋白直接结合其自身和Antenna-pedia (Antp) 基因促进器附近的DNA序列. 这种结合表明Ubx通过与特定的DNA区域相互作用来调节基因活性.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 在Drosophila中Ultrabithorax (Ubx) 基因编码控制分段身份的蛋白质.
- Ubx蛋白在确定后胸部和前腹部部分的发育命运方面发挥着至关重要的作用.
- 遗传研究表明,Ubx抑制了天线-pedia (Antp) 均质基因.
研究的目的:
- 研究Ubx蛋白调节基因表达的分子机制.
- 确定Ubx蛋白是否是DNA结合蛋白,并确定它们的DNA点.
- 阐明Ubx蛋白与目标基因的调节区域之间的直接相互作用.
主要方法:
- 从过度生产的大肠杆菌菌株中净化Ubx蛋白家族成员.
- 纯化的Ubx蛋白质作为特定序列的DNA结合蛋白质的表征.
- 鉴定和分析由Ubx蛋白结合的DNA序列,包括那些靠近Ubx和Antp促进体的DNA序列.
主要成果:
- 一个Ubx蛋白成员成功从大肠杆菌中净化.
- 纯化的Ubx蛋白显示出特定序列的DNA结合活性.
- 在Ubx基因和Antp P1促进体的促进体附近确定了结合点.
- 这些约束位,范围从40到90bp,包含TAA或TAA-TCG的双重重复.
结论:
- Ubx蛋白直接通过特定序列的DNA结合来调节基因表达.
- 鉴定到的结合部位表明了Ubx介导的转录调节的直接机制.
- 这些发现为Ubx在发育模式中的作用提供了分子洞察力.
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