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设计基本的金属依赖DNA识别和细胞内化

Soraya Learte-Aymamí1, Natalia Curado1, Jéssica Rodríguez1

  • 1Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) and Departamento de Química Orgánica, Universidade de Santiago de Compostela , 15782 Santiago de Compostela, Spain.

Journal of the American Chemical Society
|October 24, 2017
PubMed
概括

研究人员设计了一种可选择性地将DNA与结合的. 这种金属促进的相互作用是可逆的,并使进入细胞.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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