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一种追踪蛋白质聚合物和控制子遗传的遗传工具

Gregory A Newby¹, Szilvia Kiriakov², Erinc Hallacli³

¹Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.

|October 24, 2017

概括

科学家们开发出一种新的遗传工具来追踪和控制细胞中的蛋白质聚合. 这项技术有助于研究疾病和细胞功能,

科学领域:

细胞生物学
合成生物学
遗传学

背景情况:

蛋白质聚合与许多疾病和细胞功能有关.
由于缺乏高通量细胞工具,研究蛋白质聚合是具有挑战性的.

研究的目的:

开发一种用于检测和控制蛋白质聚合的合成遗传工具.
应用该工具研究酵母和RNA结合蛋白 (RBPs).

主要方法:

开发一种用于检测和控制蛋白质聚合的合成遗传工具.
用于酵母追踪聚合状态并创建合成记忆.
高通量屏幕用于识别固突变物和设计反驱动器.
扩展该技术研究酵母RNA结合蛋白 (RBPs) 的聚合.

主要成果:

成功追踪了酵母聚合状态和编码合成记忆.
鉴定了治疗突变物,并开发了从酵母群中消除的方法.
通过追踪它们的聚合倾向和共发生, 发现了一组聚合的RBP.

结论:

开发的技术为研究和控制蛋白质聚合提供了定量,高吞吐量和可通用的平台.
这种工具在了解涉及蛋白质聚合的疾病机制和细胞过程中具有广泛的应用.

关键词:

结合RNA的蛋白质表观遗传学高通量选记忆力子蛋白质聚合物合成生物学合成转录因子在 yTRAP

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