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惯性平衡显示了哺乳动物细胞中的快速质量波动
David Martínez-Martín1, Gotthold Fläschner1, Benjamin Gaub1
1Eidgenössische Technische Hochschule (ETH) Zürich, Department of Biosystems Science and Engineering, 4058 Basel, Switzerland.
Nature
|October 27, 2017
概括
活细胞在整个细胞周期中表现出微妙的,快速的质量波动, 这一发现为细胞质量调节和疾病提供了新的见解.
科学领域:
- 细胞生物学
- 生物物理
- 生理学
背景情况:
- 细胞大小,体积和质量调节对于生理功能至关重要,并且与新陈代谢,增殖和基因表达有关.
- 细胞质量的失调导致各种疾病.
- 现有的技术难以以跟踪附着细胞质量,
研究的目的:
- 开发一种新技术,以高时间和质量分辨率跟踪附着细胞质量.
- 在生理条件下研究单个附着细胞的动态质量波动.
- 探索细胞质量波动与细胞过程之间的关系.
主要方法:
- 使用光学激发的微共振器测量附着细胞质量的"平衡"的开发.
- 长时间 (天) 的高分辨率测量 (图形图灵敏度,毫秒分辨率).
- 与光显微镜相容,用于同时观察细胞过程.
主要成果:
- 活着的哺乳动物细胞在整个细胞周期中表现出1-4%的内在质量波动.
- 这些波动与ATP合成和水运输有关.
- 细胞感染疫苗病毒会阻止细胞生长和细胞循环的进展,但不会导致细胞死亡.
结论:
- 所有活细胞都在细胞周期中表现出快速,微妙的质量波动.
- 开发的"皮科平衡"技术使得我们能够对细胞质量动态有前所未有的洞察力.
- 这种方法在理解生理学,癌症,干细胞分化和药物发现中的细胞质量调节方面具有广泛的应用.
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