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相关概念视频

Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

15.9K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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pre-mRNA Processing02:01

pre-mRNA Processing

57.7K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.9K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
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细胞mRNA3'-end处理机器的架构

Ana Casañal1, Ananthanarayanan Kumar1, Chris H Hill1

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概括
此摘要是机器生成的。

研究人员阐明了酵母分裂和多化因子 (CPF) 聚合酶模块的结构. 这一发现揭示了酶如何协调处理信使RNA (mRNA) 3'端.

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科学领域:

  • 分子生物学
  • 生物化学
  • 结构生物学

背景情况:

  • 细胞前体信使RNA (mRNA前体) 经历了至关重要的3'-end处理.
  • 多蛋白分裂和多化因子 (CPF) 调解了前mRNA分裂,多化和转录终止.
  • 目前尚不清楚CPF酶活性的精确协调和组合.

研究的目的:

  • 研究酵母CPF的结构结构.
  • 通过CPF阐明mRNA3'-end处理协调的机制.
  • 要确定CPF聚合酶模块的结构.

主要方法:

  • 使用电子冷显微镜 (cryo-EM) 来确定CPF聚合酶模块的结构.
  • 为了验证功能方面,进行了体外复制实验.
  • 结构分析侧重于聚合酶模块内的蛋白质组件的排列.

主要成果:

  • 酵母CPF的核酶,聚合酶和酸酶活动分为三个不同的模块.
  • 确定了大约200千多CPF聚合酶模块的3.5安斯特罗姆分辨率结构.
  • 聚合酶模块具有四个β螺旋,类似于其他核酸结合蛋白质复合体.
  • 聚合酶模块促进了聚烯酸尾的特定和高效添加.

结论:

  • 确定的结构为CPF酶的协调作用提供了见解.
  • 聚合酶模块在聚合精确聚化所必需的因素中发挥着关键作用.
  • 这项研究提高了我们对真核生物3'-end处理调节的理解.