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通过参磁转移反应剂的帮助,CET对d-和l-乳酸盐进行反体识别

Lei Zhang¹, André F Martins^1,2, Piyu Zhao¹

¹Department of Chemistry, University of Texas at Dallas , 800 West Campbell Road, Richardson, Texas 75080, United States.

Journal of the American Chemical Society

|October 31, 2017

概括

研究人员开发了用化学交换和转移 (CEST) 图像进行选择性检测的d-和l-乳酸盐复合物. 这一进步使得癌症研究中的向代谢物成像成为可能.

科学领域:

生物医学成像
核磁共振 (NMR) 光谱学
化学生物学

背景情况:

细胞外乳酸是癌症中的关键代谢生物标志物,通常使用NMR转移试剂检测化学交换和转移 (CEST) 图像.
欧 (Eu) DOTA-三胺 (EuDO3A) 之前已经显示出对癌细胞乳酸盐产生成像的潜力.

研究的目的:

设计和评估新型的七度牙宏循环Ytterbium (Yb) DOTA-trisamide复合物,用于刻板选择乳酸检测.
通过高分辨率的NMR和CEST成像来研究这些复合物的d-乳酸盐和l-乳酸盐之间的区别潜力.

主要方法:

一系列YbDO3A-trisamide复合物的合成和表征,其中包括悬挂臂中的性中心.
高分辨率的1H核磁共振光谱研究乳酸和Yb复合体之间的相互作用.
密度函数理论 (DFT) 计算以了解立体选择复合体的形成.
在CEST的成像实验中评估了乳酸酶选择性检测的可行性.

主要成果:

通过NMR和DFT计算证实了立体选择性乳酸·YbDO3A-三胺复合物的形成.
每个复合物都表现出独特的CEST特征,使乳酸分化成为可能.
通过使用高分辨率的NMR和CEST技术,成功地区分了d-乳酸盐和l-乳酸盐.

结论:

基兰化物转移试剂,特别是YbDO3A-trisamide复合物,可以设计用于立体选择性检测乳酸盐.
这种方法可以通过CEST成像选择性检测d-和l-乳酸酶体.
这些发现突显了定制的偏磁转移试剂在生物医学应用中的向代谢物成像的潜力.

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