单细胞RNA测序揭示了疟疾寄生虫发生性关系的特征
Asaf Poran1,2,3, Christopher Nötzel4,5, Omar Aly1,2
1Institute for Computational Biomedicine, Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York 10065, USA.
Nature
|November 3, 2017
概括
疟疾寄生虫Plasmodium falciparum的性活动依赖于ap2-g的激活. 单细胞RNA测序显示ap2-g+分裂体对游戏细胞发育的表观遗传调节剂进行上调.
科学领域:
- 分子寄生病学
- 基因组学
- 表观遗传学
背景情况:
- 疟疾寄生虫需要平衡宿主持久性和传播.
- 持续性涉及无性复制,而传播则取决于雌性细胞的分化.
- 性承诺发生在特定的细胞周期中,由ap2-g激活调节.
研究的目的:
- 为了阐明在性交期间发生的Plasmodium falciparum转录变化.
- 确定AP2-G在性行为寄生虫的基因表达调节中的作用.
- 了解构造细胞发育的表观遗传机制.
主要方法:
- 超过18000个寄生虫转录组的单细胞RNA测序.
- 条件AP2-G和野生型NF54菌株的分析.
- 在多个发育阶段进行比较的转录基因分析.
主要成果:
- 经过性交,AP2-G+成熟的分裂体会提高特定的基因表达模式.
- 上调基因包括额外的AP2转录因子和表观遗传修饰剂.
- 这些调节剂参与了基质子的修饰和核酶定位.
结论:
- 在Plasmodium falciparum中,AP2-G是启动性发育的关键调节剂.
- 鉴定出的表观遗传调节剂可能促进下游基因表达.
- 了解这些机制对于控制疟疾传播的策略至关重要.
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