裂变酵母中U6小核RNA的基因有一个内置基因
Nature
|January 5, 1989
概括
研究人员在Schizosaccharomyces pombe U6小核RNA (snRNA) 基因中发现了一个内子,这是snRNA基因的首次. 这种内子被精确地去除,这表明U6RNA可能除了在mRNA前拼接中的作用外,还可以作为拼接基质.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 小核RNAs (snRNAs) 在真核细胞中对mRNA前拼接至关重要.
- 哺乳动物细胞利用U1-U6 snRNAs,形成小核核核糖核蛋白颗粒 (snRNP).
- U1,U2,U4,U5和U6 snRNP是结合体的组成部分.
研究的目的:
- 为了描述编码Schizosaccharomyces pombe U6 snRNA的基因.
- 为了研究这种酵母物种中U6 snRNA的结构和处理.
主要方法:
- 基因表征技术.基因表征技术.
- 北方斑点分析.
- RNA测序. 进行RNA测序.
主要成果:
- 奇萨克罗米切斯贝U6 snRNA基因意外地含有50个基对的内类序列.
- 北方斑点和RNA测序证实了这个内子从转录中被精确地去除.
- 成熟的Schizosaccharomyces pombe U6 RNA与哺乳动物的U6 RNA共享77%的序列相似性.
结论:
- 这是第一个在小核RNA基因内发现内子的案例.
- 这些发现表明,Schizosaccharomyces pombe中的U6RNA可能作为拼接基质,除了在mRNA前拼接中的作用外.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nonsense-mediated mRNA Decay
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Riboswitches
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The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...


