通过调节性转录因子IRF-1诱导内源的IFN-alpha和IFN-beta基因
T Fujita1, Y Kimura, M Miyamoto
1Institute for Molecular and Cellular Biology, Osaka University, Japan.
Nature
|January 19, 1989
概括
干扰素调节因子-1 (IRF-1) 可以直接诱导干扰素α和β基因. 这项研究表明,单独的IRF-1基因表达可以在没有病毒触发的哺乳动物细胞中激活静音干扰素基因.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- 干扰素 (IFN) 对于细胞生长,分化和抗病毒防御至关重要.
- 病毒感染会触发IFN-α和IFN-β基因的转录.
- 转录因子IRF-1 (干扰素调节因子-1) 最近被确定,其基因是病毒诱导的.
研究的目的:
- 研究IRF-1在诱导IFN-α和IFN-β基因中的作用.
- 为了确定IRF-1是否可以诱导IFN基因表达,而不依赖于病毒刺激.
- 通过单个转录因子证明沉默染色体基因的特定诱导.
主要方法:
- 克隆的小鼠IRF-1基因在子COS细胞中得到了表达.
- 测量了内源IFN-α和IFN-β基因表达.
- 使用IRF-1/酵母GAL4基马体转录因子来评估诱导能力.
主要成果:
- 在没有病毒存在的COS细胞中,克隆小鼠IRF-1基因的高水平表达诱导了内源IFN-alpha和IFN-beta基因.
- IRF-1/酵母 GAL4 基因转录因子也证明了诱导这些 IFN 基因的能力.
- 这代表了在哺乳动物细胞中通过单个转录因子基因转染来实现特定的,静音染色体基因诱导的潜在首次演示.
结论:
- 在IFN-α和IFN-β基因的诱导中,IRF-1起着重要作用.
- 在IFN基因诱导过程中,IRF-1的新生产生至关重要.
- 转染IRF-1基因可以特别激活哺乳动物细胞中静默的IFN基因.
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