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In-Nucleus Hi-C in Drosophila Cells
Published on: September 15, 2021
主要的核蛋白质在核和细胞质之间穿
R A Borer1, C F Lehner, H M Eppenberger
1Institut für Zellbiologie, ETH Hönggerberg, Zürich, Switzerland.
Cell
|February 10, 1989
概括
核素和B23/No38蛋白质在细胞核和细胞质之间穿,挑战了以前的假设. 这种核细胞质运输表明它在调节核活动和核糖体组件运动方面发挥了作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 核素 (92 kDa) 调节RNA聚合酶I转录和生殖前RNA结合.
- B23/No38 (38 kDa) 参与了前核体颗粒的内核包装.
- 之前,这两种蛋白质都被认为是完全核的.
研究的目的:
- 为了研究核和B23/No38蛋白的定位和运动.
- 为了确定这些核蛋白是否在核和细胞质之间穿.
- 为了探索核细胞质中这些蛋白质的运输的功能意义.
主要方法:
- 跨物种异质核分析,以监测核之间的蛋白质平衡.
- 细胞质微注射抗体,然后观察抗原介导的核积累.
- 蛋白质定位和运动的免疫检测.
主要成果:
- 证据表明,核和B23/No38在核和细胞质之间不断地穿.
- 通过细胞质介导引入抗体的抗原介导核进口的演示.
- 在细胞质中意外发现了这些主要的核蛋白质.
结论:
- 核和B23/No38蛋白质在核和细胞质之间积极穿.
- 这些穿蛋白可能在核细胞质中运输核细胞体组件中发挥作用.
- 穿蛋白的细胞质暴露可能使细胞质调节核功能.
相关概念视频
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Protein Sorting
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...

