小麦D基因组的基因组序列 Aegilops tauschii
Ming-Cheng Luo1, Yong Q Gu2, Daniela Puiu3
1Department of Plant Sciences, University of California, Davis, California, USA.
Nature
|November 17, 2017
概括
现在小麦D基因组的祖先Aegilops tauschii已经获得了基因组序列的参考质量. 这项研究揭示了它的高度重复性驱动着快速的基因组进化和基因复制.
科学领域:
- 基因组学
- 植物生物学
- 进化生物学
背景情况:
- 在六倍体小麦 (Triticum aestivum) 中,Aegilops tauschii 是 D 基因组的双倍体祖先.
- 它的大,重复的基因组阻碍了之前的参考质量测序工作.
- 没有. 在小麦的改良过程中, 豆是一种重要的遗传资源.
研究的目的:
- 为了生成Aegilops tauschii ssp的参考质量的基因组序列. 加入AL8/78.
- 研究 Ae 的基因组结构和进化动态. 这是一件很糟糕的事情.
- 了解重复序列在基因组进化中的作用.
主要方法:
- 顺序克隆基因组测序
- 全基因组射击枪测序
- 生物纳米光学基因组映射
主要成果:
- 一个高质量的基因组序列. 已经成功生成了AL8/ 78.
- 这就是A. 图西基因组表现出前所未有的高度相似的重复序列.
- 与其他植物基因组相比, 图西基因呈现出更多分散的重复基因和更快的染色体进化.
- 重组率与其他草基因组的对线性丧失相关.
结论:
- 大量的类似的重复序列在AE. 可能会导致重组错误.
- 这些错误有助于基因重复和快速的染色体结构变化,推动了快速的基因组进化.
- 这些发现为塑造草基因组的进化机制和小麦遗传改进的潜在目标提供了洞察力.
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