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控制金纳米颗粒与计算机设计的协调蛋白质的关联和分离

Matthew J Eibling¹, Christopher M MacDermaid¹, Zhaoxia Qian¹

¹Department of Chemistry, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.

Journal of the American Chemical Society

|November 18, 2017

概括

计算设计的蛋白质可逆地使用离子连接金纳米颗粒. 蛋白质长度控制了纳米粒子的分离,使得可调节的材料性能可用于先进的应用.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
蛋白质工程是指蛋白质工程.

背景情况:

纳米颗粒的生物聚合物功能化创造了多功能混合材料.
蛋白质为工程提供复杂的结构,组装和识别能力.
DNA编程提供了长度和识别,但缺乏蛋白质的多功能性.

研究的目的:

通过计算设计一种用于可逆纳米粒子组装的蛋白质.
为控制的纳米粒子分离设计蛋白质长度.
为了研究离子介导的蛋白质-纳米粒子相互作用.

主要方法:

用Zn2+协调位点进行计算蛋白质设计.
设计蛋白质对金纳米粒子表面的共价附着.
使用表面等离子共振 (SPR) 和传输电子显微镜 (TEM) 监测纳米粒子的结合和分离.
调查蛋白质结构变化和自我组装对Zn2+的反应.

主要成果:

设计的蛋白质在Zn2+结合后自组装成螺旋式同质体.
蛋白质通过Zn2+调解可逆黄金纳米粒子协会.
纳米粒子分离可以根据蛋白质长度 (螺旋式重复单位) 调节.
SPR红移和TEM证实了Zn2+依赖的纳米粒子协会和分离变化.

结论:

通过计算设计的蛋白质可以实现可调节,可逆的纳米粒子组件.
蛋白质结构,折叠,长度和离子反应可以为特定的功能进行工程.
这种方法可以精确控制纳米粒子之间的距离和材料特性.

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