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塑基因交付:纳米拓学重要

Hao Song¹, Meihua Yu¹, Yao Lu¹

¹Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, Queensland 4072, Australia.

Journal of the American Chemical Society

|November 21, 2017

概括

控制纳米颗粒表面质地显著提高了等离子体DNA的传递. 具有尖表面的兰布坦样纳米颗粒显示出优异的DNA结合和转染效率,提供对降解的保护.

科学领域:

生物技术
材料科学
纳米技术

背景情况:

塑DNA的传递对于各种生物应用至关重要.
非病毒载体对于将等离子体DNA引入细胞至关重要.
纳米粒子表面地形影响向量性能.

研究的目的:

研究纳米粒子纳米拓对等离子体DNA结合和转染功能的影响.
设计基于的新型纳米导体以有效地传递基因.
了解纳米粒子表面结构对核酶降解的保护机制.

主要方法:

具有多种形态的二氧化纳米颗粒的合成 (兰布坦,树,花样).
纳米粒子纳米拓学 (尖峰,半球,碗型子单位) 的表征.
对等离子体DNA结合能力和体外传染疗效的评估.
对核酶降解的保护的评估.

主要成果:

具有尖表面的兰布坦样纳米粒子表现出最高的等离子体DNA结合能力.
这些尖的纳米颗粒实现了88%的传染效率,超过了其他测试的形态和一些商业剂.
尖的表面结构通过多价值相互作用为DNA结合提供了连续的空间,并保护DNA免受核酶降解,防止转染衰变.

结论:

控制纳米颗粒的纳米拓学是提高基因传递效率的关键因素.
具有尖表面的兰布坦样纳米粒子代表了有效和稳定的等离子体DNA传递的有希望的非病毒载体.
这项研究为先进的非病毒基因传递系统提供了理性的设计原则.

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Plasmids

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...