正角肌肉纤维在平面再生中具有不同的指导作用
M Lucila Scimone1,2,3, Lauren E Cote1,2,3, Peter W Reddien1,2,3
1Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Nature
|November 24, 2017
概括
平面肌肉纤维在再生过程中起着不同的作用. 通过myoD和nkx1-1等基因调节的特定肌肉类型对于在再生过程中启动和塑造新身体部位至关重要.
科学领域:
- 发育生物学
- 复原医学
- 平面生物
背景情况:
- 在动物中再生是常见的,但位置信息在差异化组织中的作用仍然不清楚.
- 平面再生研究已经确定了参与模式的位置控制基因 (PCG),通常在肌肉中表达.
- 平面肌肉的特征和不同肌肉子集在再生中的功能作用尚不清楚.
研究的目的:
- 研究不同类型的肌肉纤维在平面再生中的不同调节作用.
- 确定特定肌肉子集的基因基础.
- 了解肌肉特征如何影响伤口信号和前后轴模式.
主要方法:
- 在平面生物中抑制基因表达的RNA干扰 (RNAi).
- 由基因抑制引起的再生表型的特征.
- 基因表达模式的分析,包括myoD和nkx1-1.
- 显微镜检查肌肉纤维的形成和方向.
主要成果:
- 基因myoD对于形成纵向肌肉纤维至关重要,对于再生启动至关重要.
- 纵向纤维的损失导致完全的再生失败.
- 基因nkx1-1是循环肌肉纤维形成的必需基因,影响前后轴的模式.
- 圆形纤维的损失导致轴形成的缺陷,例如分叉轴和合头.
结论:
- 平面动物的不同类型的肌肉纤维在再生过程中具有不同的调节功能.
- 特定的肌肉子集由myoD和nkx1-1等基因控制, 对于伤口信号和模式至关重要.
- 肌肉不仅可以收缩,而且在调节再生过程中发挥重要作用.
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