在CRISPR生物磁带上随着时间的推移记录细胞事件
Ravi U Sheth1,2, Sung Sun Yim1, Felix L Wu1,2
1Department of Systems Biology, Columbia University, New York, NY, USA.
Science (New York, N.Y.)
|November 25, 2017
概括
科学家们使用CRISPR-Cas创建了一个生物磁带记录器, 这可以准确地重建过去的细胞事件和环境变化.
科学领域:
- 合成生物学
- 基因组学
- 分子生物学
背景情况:
- 生物过程是动态的,但对时间变化的信号和监管程序进行分析是具有挑战性的.
- 准确的细胞状态和环境因素的时间测量对于理解生物系统至关重要.
研究的目的:
- 在细胞基因组中直接记录时间生物信息的新框架.
- 创造一个能够稳定,长期记录和准确重建过去生物事件的系统.
主要方法:
- 在生物信号诱导细胞内DNA生产的"生物磁带录音机".
- 使用CRISPR-Cas适应系统记录基因组中的DNA.
- 测序CRISPR阵列以重建时间和血统信息.
- 实施多重复合策略,同时记录多个环境代谢物.
主要成果:
- 在多天内稳定记录生物信息.
- 从被测序的CRISPR阵列中准确地重建时间和血统数据.
- 成功多重记录了三种不同的代谢物 (铜,三糖,糖).
- 验证了测量细胞动态状态和环境变化的能力.
结论:
- 生物磁带记录器提供了一种可靠的方法来记录时间生物信息.
- 这项技术可以对生物事件进行大规模的长期记录.
- 开辟了研究动态生物过程和环境相互作用的新途径.
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