一种储存和检索更多遗传信息的半合成生物
Yorke Zhang1, Jerod L Ptacin2, Emil C Fischer1
1Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA.
Nature
|December 1, 2017
概括
科学家们创造了一种半合成的生物体, 具有扩展的DNA字母, 能够存储和检索更多的遗传信息. 这一突破推动了合成生物学,
科学领域:
- 合成生物学
- 分子生物学
- 遗传学
背景情况:
- 生命的基因信息依赖于四个字母的DNA字母和两个基因对.
- 合成生物学旨在通过扩展这种遗传系统来创造新的生命形式.
- 在之前的研究中,在大肠杆菌中发现了具有非自然基因对 (dNaM-dTPT3) 的半合成生物.
研究的目的:
- 展示一个扩展的遗传代码的体内转录和翻译.
- 通过非自然的基对来实现特定位点的氨基酸结合.
- 建立一个创建新生物功能的平台.
主要方法:
- 这种大肠杆菌进口并复制含有不自然基因对dNaM-dTPT3的DNA.
- 在体内实现非自然DNA编码子转化为mRNA.
- 使用相关的tRNA和核糖体进行氨基酸结合的非自然编码子的翻译.
主要成果:
- 通过dNaM-dTPT3成功转录到含有非自然编码子的mRNA.
- 通过特定的tRNA展示了非自然的核糖体解码.
- 将自然和非正规氨基酸集成到超级绿色光蛋白中.
结论:
- 不自然的基对可以在体内有效地转录和翻译.
- 非键相互作用对于遗传信息的存储和检索至关重要.
- 这种扩展的遗传系统为新型合成生命形式和功能提供了基础.
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