下一代连接地图:L1000平台和第一个100万个配置文件
Aravind Subramanian1, Rajiv Narayan1, Steven M Corsello2
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Cell
|December 2, 2017
概括
连接地图 (CMap) 使用L1000分析方法扩大了1000倍. 这种扩展的CMap将基因,药物和疾病通过基因表达签名连接起来,用于药物发现和临床应用.
科学领域:
- 基因组学
- 系统生物学
- 药物基因组学
背景情况:
- 连接地图 (CMap) 通过基因表达签名连接生物状态.
- 之前的CMap版本的规模和范围有限.
研究的目的:
- 报告使用L1000技术扩大CMap的1000倍.
- 证明扩展CMap对生物发现和临床应用的有用性.
主要方法:
- 开发并实施一种名为L1000的低成本,高吞吐量减少表达式分析方法.
- 作为NIH LINCS联盟的一部分,生成了超过130万个L1000配置文件.
- 验证了L1000的可复制性及其计算推断未测量的转录表达的能力.
主要成果:
- 扩展的CMap显著增加了可用的基因表达数据的规模.
- L1000分析具有很高的可重现性,与RNA测序相似.
- 计算推断成功预测了81%的非测量转录的表达水平.
- 扩展的CMap有助于发现小分子的作用机制.
- 它有助于在疾病基因中的基因变异的功能注释.
- 根据临床试验设计和患者分层.
结论:
- 这种由L1000驱动的扩展型CMap是系统药理学的重大进步.
- 这种资源为药物机制,疾病遗传学和个性化医疗提供了新的洞察力.
- 这些数据和分析工具在https://clue.io上公开提供.
相关概念视频
Maximum Power Flow and Line Loadability
631
The maximum power flow for lossy transmission lines is derived using ABCD parameters in phasor form. These parameters create a matrix relationship between the sending-end and receiving-end voltages and currents, allowing the determination of the receiving-end current. This relationship facilitates calculating the complex power delivered to the receiving end, from which real and reactive power components are derived.
631
Distributed Loads
1.0K
Distributed loads are a common type of load that engineers and scientists encounter in various practical situations. Distributed loads often refer to a type of load spread over a surface or a structure and can be modeled as continuous force per unit area.
For example, consider a bookshelf filled with books stacked vertically adjacent to each other. The weight of the books is evenly distributed over the length of the shelf. As a result, the pressure at different locations on the surface of the...
For example, consider a bookshelf filled with books stacked vertically adjacent to each other. The weight of the books is evenly distributed over the length of the shelf. As a result, the pressure at different locations on the surface of the...
1.0K
Network Function of a Circuit
909
Frequency response analysis in electrical circuits provides vital insights into a circuit's behavior as the frequency of the input signal changes. The transfer function, a mathematical tool, is instrumental in understanding this behavior. It defines the relationship between phasor output and input and comes in four types: voltage gain, current gain, transfer impedance, and transfer admittance. The critical components of the transfer function are the poles and zeros.
909
Bus Impedance Matrix
542
Calculating subtransient fault currents for three-phase faults in an N-bus power system involves using the positive-sequence network. When a three-phase short circuit occurs at a specific bus, the analysis uses the superposition method to evaluate two separate circuits.
In the first circuit, all machine voltage sources are short-circuited, leaving only the prefault voltage source at the fault location. The positive-sequence bus impedance matrix can be determined by solving the nodal equations,...
In the first circuit, all machine voltage sources are short-circuited, leaving only the prefault voltage source at the fault location. The positive-sequence bus impedance matrix can be determined by solving the nodal equations,...
542
Profile Leveling and Cross Sections
1.7K
Profile leveling and cross-sections are surveying methods used to determine and document terrain elevations for infrastructure projects such as highways, railroads, canals, and pipelines. These methods provide data for earthwork planning and alignment of proposed routes. Profile leveling involves measuring elevations along a fixed line to create a vertical terrain profile. A surveyor sets up a leveling instrument at the benchmark (BM) and records a backsight (BS) to determine the...
1.7K
Power System Distribution
1.1K
Power system distribution involves delivering electrical energy from power plants to consumers through a network of transmission and distribution systems. The process begins at power plants, where energy from coal, gas, nuclear, water, and wind is converted into electrical energy. These plants use three-phase generators, typically rated between 50 to 1300 MVA, with terminal voltages ranging from a few kV to 20 kV, depending on the size and age of the units.
The transmission system is designed...
The transmission system is designed...
1.1K


