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头癌中原发性和转移性瘤生态系统的单细胞转录组分析

Sidharth V Puram¹, Itay Tirosh², Anuraag S Parikh¹

¹Department of Pathology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA; Department of Otology and Laryngology, Harvard Medical School, Boston, MA 02115, USA.

|December 5, 2017

概括

这项研究揭示了头部和部状细胞癌 (HNSCC) 细胞的部分上皮转移 (p-EMT) 预测转移. 了解瘤细胞异质性和结构相互作用是HNSCC治疗的关键.

科学领域:

癌症学
基因组学
细胞生物学

背景情况:

瘤微环境的组成,包括恶性,层状和免疫细胞,影响癌症的进展和治疗结果.
头部和部状细胞癌 (HNSCC) 是一种复杂的疾病,细胞异质性会影响患者的预后.

研究的目的:

在单细胞分辨率下分析HNSCC的细胞生态系统.
为了识别恶性和体内不同的细胞程序.
研究部分上皮细胞转移到介质细胞转移 (p-EMT) 在HNSCC转移和临床特征中的作用.

主要方法:

来自18名HNSCC患者的约6,000个单细胞RNA测序,包括原发性瘤和淋巴结转移.
整合单细胞数据与来自更大的HNSCC瘤队列的批量表达特征.
在瘤组织中的p-EMT表达细胞的空间分析.

主要成果:

在HNSCC患者中,肌体和免疫细胞表现出保存的表达模式.
恶性细胞表现出与细胞周期,压力,缺氧,分化和p-EMT相关的显著异质性.
在主要瘤的前端主要观察到p-EMT特征.
确定p-EMT是结节转移,瘤等级和不良病理特征的独立预测因子.
根据恶性细胞和树皮细胞组成的精细HNSCC亚型.

结论:

单细胞分析提供了对HNSCC细胞生态系统的关键见解.
流体相互作用和p-EMT程序与HNSCC的转移潜力有显著的关联.
了解细胞异质性和p-EMT等特定程序可以完善HNSCC分类并确定治疗点.

关键词:

从上皮细胞转变为介质细胞头部和部状细胞癌瘤内异质性发生转移 scRNA-seq 在单细胞RNA测序瘤微环境

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