Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Structure01:23

RNA Structure

79.3K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.3K
RNA Structure01:19

RNA Structure

7.8K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.8K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.5K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.5K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.8K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.8K
The DNA Replication Fork01:02

The DNA Replication Fork

41.3K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.3K
Nucleic acids02:43

Nucleic acids

194.2K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
194.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Intersegmental transfers drive target search in an RNA-targeting CRISPR system.

bioRxiv : the preprint server for biology·2026
Same author

A ROS-Responsive DNA Nanodevice for Targeted Cytosolic siRNA Delivery in Metabolic Dysfunction-Associated Steatohepatitis.

Journal of the American Chemical Society·2026
Same author

Integrating Divergence-Based Proteomic Analysis and Directed Network Diffusion to Characterize Diagnosis-Anchored Molecular Variability at the Metabolic Syndrome-Migraine Interface.

International journal of molecular sciences·2026
Same author

Multimodal control of Cas13d activity through domain insertion at an allosteric hotspot.

Nature communications·2026
Same author

Towards deployable CRISPR-based nucleic acid detection.

Progress in biomedical engineering (Bristol, England)·2026
Same author

A synthetic cell microreactor with two types of interacting dynamic DNA-based pores.

Nature chemistry·2026

相关实验视频

Updated: Feb 16, 2026

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
08:59

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

12.2K

单链DNA和RNA原

Dongran Han1,2, Xiaodong Qi3,4, Cameron Myhrvold1,2

  • 1Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.

Science (New York, N.Y.)
|December 16, 2017
PubMed
概括

研究人员开发了一种设计单一DNA或RNA链的新方法, 这一进步使得可扩展的自下而上的纳米技术具有可复制的核酸纳米结构.

更多相关视频

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.1K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

12.2K

相关实验视频

Last Updated: Feb 16, 2026

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
08:59

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

12.2K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.1K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

12.2K

科学领域:

  • 生物技术
  • 纳米技术
  • 合成生物学

背景情况:

  • 聚合物的自我折叠成定义的结构在生物学中至关重要.
  • 多组件自组合已经创造了复杂的合成纳米结构.
  • 单分子折叠策略在复杂性和可扩展性方面面临限制.

研究的目的:

  • 建立一个设计和合成单核酸链的框架,这些链可以自行折叠成任意,复杂和无结的形状.
  • 证明单分子折叠的可行性,用于制造大规模的可复制纳米结构.

主要方法:

  • 开发一个计算框架来预测和设计单分子折叠路径.
  • 多基基单链DNA和RNA结构的实验合成.
  • 在体外和体内 (活细胞) 复制设计的核酸链.

主要成果:

  • 成功设计和实验建造多样化,复杂和未结合的单链核酸纳米结构.
  • 创建一个~10,000核酸的DNA结构和一个~6000核酸的RNA结构.
  • 证明这些结构在体外和活细胞内容易复制.

结论:

  • 单分子折叠是构建复杂和可复制的核酸纳米结构的可行和通用策略.
  • 这种方法显著扩大了自下而上的纳米技术的设计空间和材料可扩展性.
  • 开发的框架有助于创建定制的核酸纳米材料.