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视觉化60S前核核核糖体的组合路径
Lukas Kater1, Matthias Thoms2, Clara Barrio-Garcia1
1Gene Center Munich and Center of Integrated Protein Science-Munich (CiPS-M), Department of Biochemistry, Feodor-Lynen-Str. 25, University of Munich, 81377 Munich, Germany.
Cell
|December 16, 2017
概括
这项研究使用冷电子显微镜揭示了真核中60S核糖体子单元组合的早期步骤. 它详细介绍了结构途径,并确定了参与核糖体生物发生的关键蛋白质复合体.
科学领域:
- 分子生物学
- 结构生物学
- 细胞生物学
背景情况:
- 细胞60S核糖体子单元生物发生是一个复杂的过程,发生在细胞核中.
- 对于早期组装中间产品的结构数据有限,这阻碍了对这一关键途径的理解.
研究的目的:
- 阐明早期60S核糖体子单元的结构路径.
- 识别和绘制组装因子在核糖体生物发生中的作用.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 来确定早期60S生物生成中间体的结构.
- 从3.3 Å到4.5 Å的高分辨率结构得到.
主要成果:
- 在六个状态中确定了明显的不成熟的rRNA构造和25个组装因子.
- Nsa1-Rrp1-Rpf1-Mak16模块稳定了子单元的溶剂侧.
- Erb1-Ytm1-Nop7复合体组织了多个因子和rRNA域.
结论:
- 结构快照显示了主要60S领域的逐步整合和紧缩.
- 组装从出口道周围的溶剂侧向中心突起进行.
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