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蛋白质中的酸盐-水网络的缓慢动态

R Bryn Fenwick¹, David Oyen¹, H Jane Dyson¹

¹The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

Journal of the American Chemical Society

|December 20, 2017

概括

蛋白质结合的水动力学会影响生物分子功能. 核磁共振研究揭示了蛋白质构造变化期间的水网络几何变化,影响了生物过程.

科学领域:

生物化学
结构生物学
生物物理

背景情况:

水对生物分子动力学和生物过程有重大影响.
微秒到毫秒 (μs-ms) 时间尺度上的蛋白质构造变化与蛋白质功能有关.
蛋白质结合的水在这些功能性结构变化过程中的行为仍然不太清楚.

研究的目的:

研究蛋白质结合的水在功能结构变化中的作用和动态.
通过使用NMR放松分散测量间接探测水网络动态.
了解水如何在结构变化过程中调解蛋白质区域之间的通信.

主要方法:

使用NMR放松分散测量来研究二叶酸还原酶 (DHFR) 中的三甲醇.
在μs-ms时间尺度结构变化过程中分析了内NH质子和环原子的化学变化.
综合实验化学转移与密度函数理论 (DFT) 的预测.

主要成果:

在结构转变过程中观察到印NH质子的显著化学转移变化,但对环的变化较小.
证明三英NH质子保持水结合,而周围的水网络几何变化.
确定放松分散可以间接地报告水动力学.

结论:

在μs-ms蛋白质构造变化过程中,蛋白质结合的水体几何变化是动态的.
水分子在功能重要蛋白质区域中传输信息方面发挥着关键作用.
蛋白质内部的运动可以通过蛋白质结构中的结水网络进行合.

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