来自寄生植物Cuscuta campestris的微RNA目标宿主信使RNA
Saima Shahid1,2, Gunjune Kim3, Nathan R Johnson1,2
1Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Nature
|January 5, 2018
概括
多德寄生植物使用微RNA (miRNA) 调节宿主基因表达. 这些植物衍生的miRNA向宿主mRNA,影响基因沉默并促进寄生虫的生长.
科学领域:
- 植物生物学
- 遗传学
- 分子生物学
背景情况:
- 鱼 (Cuscuta spp.) 其他 是使用haustoria连接宿主植物的寄生虫.
- 在宿主和寄生虫之间促进分子的转移,包括微RNA (miRNA).
- 这些转移的分子,特别是miRNAs在植物寄生病中的功能作用仍然在很大程度上未知.
研究的目的:
- 研究从Cuscuta campestris转移到宿主植物的微RNA (miRNA) 的作用.
- 确定这些miRNA是否可以调节宿主基因表达并影响寄生虫相互作用.
- 探索这些miRNA作为植物寄生虫的毒性因素的潜力.
主要方法:
- 在Arabidopsis thaliana寄生期间对Cuscuta campestris haustoria中微RNA (miRNA) 积累的分析.
- 通过C. campestris衍生的miRNAs调节的A. thaliana中的目标信使RNAs (mRNAs) 的鉴定.
- 评估miRNA向对宿主基因表达和二次小干扰RNA (siRNA) 生产的影响.
- 在A. thaliana突变体上对C. campestris生长的评估
- 在C. campestris寄生 Nicotiana benthamiana中对miRNA活性进行比较分析.
主要成果:
- 在寄生期间,Cuscuta campestris haustoria会积累高水平的22核酸微RNA (miRNA).
- 这些C. campestrismiRNA向并分裂特定的A. thalianamRNA,导致mRNA积累减少和二次小干扰RNA (siRNA) 的产生.
- 在A. thaliana的目标mRNA位点中发生的突变显著增强了C. campestris的生长.
- 已识别的miRNAs在不同的宿主物种中保存和活跃,包括Nicotiana benthamiana.
- 其他植物物种的目标mRNA同类物质含有C. campestris miRNA的预测目标部位.
结论:
- 在寄生期间,Cuscuta campestris利用特定的microRNAs (miRNAs) 来操纵宿主基因表达.
- 这些植物衍生的miRNAs作为跨物种调节剂,准宿主mRNA,并可能作为毒性因子.
- 这些发现揭示了由跨物种miRNA调节介导的植物寄生虫相互作用的新机制.
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