用于探索蛋白质功能格局的乳液中的多重复合基因合成
Calin Plesa1, Angus M Sidore2, Nathan B Lubock1
1Department of Chemistry and Biochemistry, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.
概括
我们开发了DropSynth, 这是一种新方法, 以便于建造成千上万个合成基因. 这种工具可以对基因功能进行大规模测试,加速生物发现.
科学领域:
- 合成生物学
- 分子生物学
- 基因组学
背景情况:
- 在生物研究中,有效的DNA序列构造和功能性表征至关重要.
- 目前用于大规模基因合成和功能测试的方法可能昂贵且耗时.
研究的目的:
- 介绍DropSynth,一种可扩展,低成本的基因长度DNA结构的聚合,多重合成方法.
- 通过分析基因同类函数来证明DropSynth对大规模功能基因组学的实用性.
主要方法:
- DropSynth使用条码珠捕获基因组装所需的寡核酸.
- 氧核酸被加工并组装成油水乳液中的基因结构.
- 进行多重复合功能测试以测试合成基因在* Escherichia coli*中的补充活性.
主要成果:
- 通过使用DropSynth成功合成了超过7000个定义的基因长度DNA结构.
- 确定了重要的大肠杆菌基因的基因组学多样性.
- 通过多重测试确定基因同类基因功能不相容的核心功能动机和原因.
结论:
- DropSynth为合成基因构建提供了一个可扩展和具有成本效益的平台.
- 通过将DropSynth与多重功能测试相结合,可以进行前所未有的序列功能关系探索.
- 这种方法加快了对基因功能和进化的理解.
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