在转移性黑色素瘤患者中,开始性微生物组与抗PD-1疗效有关
Vyara Matson1, Jessica Fessler1, Riyue Bao2,3
1Department of Pathology, University of Chicago, Chicago, IL 60637, USA.
概括
肠道微生物群影响癌症免疫疗法. 在响应者中大量存在的Bifidobacterium longum等特定细菌可能会提高黑色素瘤患者的抗PD-1治疗效率.
科学领域:
- 癌症学
- 免疫学
- 微生物组研究
背景情况:
- 抗编程细胞死亡蛋白1 (抗PD-1) 免疫疗法是癌症的关键治疗方法,但患者的反应率有显著差异.
- 免疫疗法的患者间异质性可能与肠道微生物组合的差异有关.
- 临床前模型表明微生物组在调节抗瘤免疫力和治疗效果方面的作用.
研究的目的:
- 对转移性黑色素瘤患者的抗PD-1 免疫疗法的临床反应进行研究.
- 确定与免疫疗法的良好反应相关的特定细菌物种.
主要方法:
- 使用16S rRNA基因测序和转基因枪测序对转移性黑色素瘤患者的初始便样本进行分析.
- 用量化聚合酶链反应 (qPCR) 来定位细菌的数量.
- 对无菌小鼠进行便微生物移植以评估功能影响.
主要成果:
- 对抗PD-1治疗的临床反应和开始时的微生物组成之间发现了显著的相关性.
- 对治疗有反应的患者中,比如Bifidobacterium longum,Collinsella aerofaciens和Enterococcus faecium等细菌物种的数量更为丰富.
- 从响应者转移到无菌小鼠的便物增强了瘤控制,T细胞反应和抗PD-L1疗法的有效性.
结论:
- 肠道微生物组在影响抗瘤免疫力和抗PD-1免疫疗法的有效性方面发挥着机械作用.
- 特定的开始性细菌可以作为预测生物标志物或治疗点,以改善免疫治疗结果.
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