一个细胞外网络的阿拉比多丰富的重复受体激酶
Elwira Smakowska-Luzan1, G Adam Mott2, Katarzyna Parys1
1Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr Bohr-Gasse 3, 1030 Vienna, Austria.
Nature
|January 11, 2018
概括
科学家绘制了植物细胞表面的相互作用,以了解富含白的重复受体激酶 (LRR-RKs) 如何形成网络. 这揭示了植物如何通过复杂的受体相互作用平衡生长和免疫信号.
科学领域:
- 植物分子生物学
- 细胞信号传输
- 系统生物学
背景情况:
- 细胞表面受体使用细胞外域 (ECD) 进行信号传递,但它们的相互作用很难研究.
- 植物具有大量的受体家族,如225个富含白的重复受体激酶 (LRR-RKs),使相互作用研究复杂化.
- 了解LRR-RK网络组织对于植物发育和免疫是至关重要的.
研究的目的:
- 调查Arabidopsis thaliana中LRR-RK的系统级组织.
- 创建细胞表面LRR-RK相互作用的全面地图.
- 展示该网络用于预测LRR-RK函数的实用性.
主要方法:
- 用一种高通量交互测试来选40,000个潜在的ECD交互.
- 建立了一个基于LRR的细胞表面相互作用网络 (CSI).
- 用生物信息预测和实验验证来确定LRR-RK功能.
主要成果:
- 发现了567个LRR- RK相互作用 (CSILRR) 的网络.
- 这项研究预测并验证了以前未经表征的LRR-RKs在植物生长和免疫方面的功能.
- 关键的LRR-RK被证明可以保持网络完整性,并防止远程受体的异常信号传输.
结论:
- 植物受体激酶网络为了解植物受体激酶信号提供了强大的工具.
- 植物利用有组织的LRR-RK网络将细胞外信号处理成平衡的生理反应.
- 这种网络组织对于调节植物生长和免疫功能至关重要.
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