分支的酶分裂以向线粒体
Hongjian He1, Jiaqing Wang1, Huaimin Wang1
1Department of Chemistry, Brandeis University , Waltham, Massachusetts 02454, United States.
Journal of the American Chemical Society
|January 13, 2018
概括
这项研究引入了一种针对线粒体的新方法,使用负电荷,在酶分裂后自组成纳米纤维. 这种方法可以有效地将载荷传递到线粒体,克服当前的脂性,性分子的局限性.
科学领域:
- 生物医学工程
- 纳米技术
- 细胞生物学
背景情况:
- 向线粒体的分子通常是脂性和阴性,具有细胞毒性风险.
- 生物医学需要更安全,更有效的线粒体向策略.
研究的目的:
- 开发一种用于向线粒体的新型酶自组系统.
- 为了证明这种系统对细胞内载荷到线粒体的有效性.
主要方法:
- 将FLAG标签与自组装图案结合在一起,形成.
- 通过肠酶 (ENTK) 诱导FLAG基因的酶分裂以诱导转化为纳米纤维.
- 细胞吸收研究和货物交付评估.
主要成果:
- 在细胞内ENTK的作用下,细胞转化为纳米纤维,主要局限于线粒体.
- 在2小时内,微粒传递的货物有效地向线粒体.
- 抑制ENTK蛋白质分解显著降低了线粒体的向.
结论:
- 酶的自我组合提供了一个基本的新策略来准亚细胞器官.
- 这种方法提供了一种更安全的替代品,即化,化线粒体向剂.
- 该系统显示了先进的细胞内药物和蛋白质传递到线粒体的潜力.
更多相关视频
07:53A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
Published on: January 9, 2019
33.9K
08:03Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
Published on: March 7, 2025
980
相关概念视频
Cleavage and Blastulation
50.5K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
50.5K
Peroxisomes and Mitochondria
98.3K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
98.3K
Peptide Bonds
83.7K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
83.7K
Mitochondria
20.7K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
20.7K
Translocation of Proteins into the Mitochondria
13.5K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.5K
Oxidative Cleavage of Alkenes: Ozonolysis
13.1K
In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
13.1K
