西方饮食触发NLRP3-依赖的天生的免疫重编程
Anette Christ1, Patrick Günther2, Mario A R Lauterbach3
1Institute of Innate Immunity, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany; Department of Infectious Diseases & Immunology, UMass Medical School, Worcester, MA 01605, USA.
Cell
|January 13, 2018
概括
无菌的炎症,比如西方饮食的炎症, 可以重新编程天生的免疫细胞, 这种重编程增强了免疫细胞的反应和增殖,可能导致炎症性疾病.
科学领域:
- 免疫学
- 表观遗传学
- 代谢疾病
背景情况:
- 训练有素的免疫包括长期的表观遗传重新编程天生的免疫细胞,增强对二次感染的反应.
- 无菌炎症触发者的作用,如饮食诱导的,在启动训练免疫力方面尚未完全理解.
研究的目的:
- 调查无菌的炎症触发剂是否会诱导受训免疫力.
- 探索饮食诱导的训练免疫机制及其对骨髓细胞功能的影响.
主要方法:
- 在Ldlr-/-小鼠中使用西方饮食 (WD) 诱导全身炎症.
- 分析了骨髓细胞反应,转录和表观遗传学重编程.
- 用氧化低密度脂蛋白 (oxLDL) 和脂聚糖 (LPS) 训练的人类单细胞进行定量特征位 (QTL) 分析.
- 使用NLRP3-/-/Ldlr-/-小鼠来评估NLRP3炎症酶的作用.
主要成果:
- 即使在改变饮食后,WD饮食也引起了全身炎症和长期增强的骨髓细胞反应.
- 在骨髓原生细胞中诱导转录基因和表观基因重编程,增加增殖和先天免疫反应.
- QTL分析和NLRP3-/-/Ldlr-/-小鼠数据表明NLRP3炎症因介导WD诱导的训练免疫,炎症和骨髓原体的增殖.
结论:
- 西方饮食引起的无菌性炎症会触发骨髓细胞中训练有素的免疫力.
- NLRP3炎症酶是饮食诱导的训练免疫和相关的骨髓细胞重编程的关键媒介.
- 这种NLRP3介导的训练免疫可能有助于训练免疫在炎症疾病中的有害影响.
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