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在Chaperonin反应中的GroEL环分离和交换

Xiao Yan¹, Qiaoyun Shi¹, Andreas Bracher¹

¹Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.

|January 17, 2018

概括

细菌的辅导体GroEL (全程) 和GroES (全程) 使用ATP将蛋白质结合并折叠. 在GroEL中暂时的环分离对于有效的蛋白质折叠和细菌生长至关重要.

科学领域:

分子生物学
蛋白质折叠
生物化学

背景情况:

细菌的陪伴剂GroEL及其辅因子GroES形成了蛋白质折叠的纳米.
GroEL和GroES在ATP调节的循环中相互作用,打开和关闭这个折叠.
GroEL是由两个堆叠的七度环组成的.

研究的目的:

研究GroEL环动态的机制.
确定短暂环分离在沙佩罗宁机制中的作用.
分析受损环分离的功能后果.

主要方法:

研究GroEL复合物的形成和动态的生物化学测试.
具有改变环分离特性的GroEL突变的分析.
基质蛋白结合和释放动态的评估.
使用大肠杆菌进行细菌生长测试.

主要成果:

在转环与ATP结合时,GroEL经历了短暂的环分离,由环间负驱动.
在环分离中缺陷的GroEL突变会形成稳定的对称GroEL:GroES2复合体.
这种突变体表现出低效的基质蛋白结合和释放.
环分离受损导致大肠杆菌生长的减少.

结论:

过渡环分离是细菌伴侣GroEL/GroES机制的一个关键,不可或缺的步骤.
这种动态过程促进了连续的,而不是同时的环功能,以实现高效的蛋白质折叠.
破坏环分离会损害沙佩罗宁的功能和细菌的活力.

关键词:

美国其他货物美国美国其他类型陪伴者这是一个很好的例子.双色光交叉相关谱学光相关谱学光共振能量转移蛋白质折叠停止流量光谱

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