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探测c-MYC i-Motif的离子大气和水分
Lutan Liu1, Byul G Kim1, Ujala Feroze1
1Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto , 144 College Street, Toronto, Ontario M5S 3M2, Canada.
Journal of the American Chemical Society
|January 23, 2018
概括
对于基因组调节和疾病至关重要的DNAi-motifs在没有体积或压缩性变化的情况下形成. 它们的形成释放出反离子,改变DNA
科学领域:
- 生物化学
- 结构生物学
- 基因组学
背景情况:
- 非正规的DNA结构,如G-四重复和i-动机,在基因组中起着调节作用,并与疾病有关.
- 了解稳定这些结构的力量是控制它们在特定基因组位点的关键.
研究的目的:
- 来自人类c-MYC瘤基因促进体的富含C的DNA序列中的i-基因形成过程中的水分和离子大气的变化.
- 在高压下使用pH依赖的声学,密度和紫外线化实验来描述i-motif形成的物理性质.
主要方法:
- 根据pH值进行声学和密度测量
- 在高压下进行紫外线化实验.
- 对形成 (ODN) 和非形成 (ODN1,ODN2) DNA 序列的比较分析.
主要成果:
- 通过c-MYC促进器DNA (ODN) 形成的i动因并没有显著改变体积或压缩性.
- 体积和压缩性数据表明内在和水分贡献之间的补偿.
- 在i-motif形成过程中,细胞蛋白质突变导致大多数反离子的释放,从而减少了DNA的多电解质性质.
结论:
- 形成DNAi-图案涉及水合和内在结构变化的复杂相互作用,导致没有净体积变化.
- 在i-motif形成过程中释放的对子离子对DNA-蛋白相互作用和基因组调节有重大影响.
- 这些发现为i-motif结构的生物物理基础及其在疾病中的潜在作用提供了洞察力.
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