通过定制的Pt (II) 两性体形成发光病毒样粒子
Stephan Sinn1, Liulin Yang2, Frank Biedermann
1Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg & CNRS , 8 Rue Gaspard Monge, 67000 Strasbourg, France.
Journal of the American Chemical Society
|January 23, 2018
概括
研究人员从复合物中制造出类似病毒的发光粒子 (VLP). 这些自组装的VLP可以模板或封装牛豆斑病毒蛋白质,形成先进材料的各种形状.
科学领域:
- 超分子化学
- 材料科学
- 生物技术
背景情况:
- 类似病毒的粒子 (VLP) 是类似病毒的蛋白质结构,但不具有传染性.
- 分子在溶液中的自组可以导致复杂的纳米结构.
- 发光金属复合体为先进的成像和传感应用提供了潜力.
研究的目的:
- 设计和制造光发病毒样粒子 (VLP) 使用 (II) 复杂的两.
- 调查这些VLP的模板和封装能力与牛菌斑病毒囊蛋白.
- 建立分子结构和VLP形态之间的关系.
主要方法:
- 发光的Pt (II) 复合两生物的自下而上的分子设计.
- 在水溶液中的超分子组合.
- 使用牛菌斑病毒体蛋白质模拟形成VLP.
- 使用传输电子显微镜 (TEM),扫描传输电子显微镜 (STEM),快速蛋白质液体染色学 (FPLC),动态光散射 (DLS) 和光物理测量进行表征.
主要成果:
- 成功创建了二面体和非二面体 (棒状) 的发光VLP.
- 根据Pt(II) 复合物的分子结构,对VLP的形状和大小进行了证明.
- 在VLPs中观察到自组装的Pt (II) 复合体的强烈发光.
- 由于蛋白质内的限制效应, 证实了增强的排放量子产量.
结论:
- 量身定制的超分子系统可以有效地控制生物构件的组装.
- 基于光发性Pt(II) 复合体的VLP提供了创建混合纳米材料的多功能平台.
- 这些混合材料对纳米技术和生物成像的应用具有前景.
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