牛血清黄金复合体的形态变化诱导的光
1Department of Physics and Optical Science, Center for Biomedical Engineering & Science, The University of North Carolina , Charlotte, North Carolina 28223, United States.
Journal of the American Chemical Society
|January 24, 2018
概括
牛血清白金 (BSA-Au) 复合体中的红色光源来自老化BSA构成的特定阴阳金结合点,而不是Au25纳米集群. 这一发现为BSA-Au复杂光机制提供了新的视角.
科学领域:
- 生物化学
- 材料科学
- 纳米技术
背景情况:
- 牛血清白蛋白 (BSA) - 金 (Au) 化合物以前被称为Au25纳米集群.
- 这些化合物可降解为纳米粒子,表明它们是BSA-cation Au复合体.
研究的目的:
- 研究BSA构成与BSA-Au复合物的光之间的关系.
- 在BSA中识别潜在的阴离子AU结合点.
- 为BSA-Au复合体的光机制提供另一种解释.
主要方法:
- 检查了pH诱导和变质的BSA结构.
- 测量了BSA-Au复合物的光特性.
- 使用激发发射地图测量.
- 确定了红色光的最低AU (III) / BSA比率.
主要成果:
- 红色光 (λem = 640 nm) 仅在老化后的 BSA 形状 (pH > 10) 中观察到.
- 其他BSA形状 (扩展,快速,正常,基本) 没有产生红色光.
- 内部能量传输机制可能会导致红色光.
- 红色光所需的Au (III) 的最小组合比小于7.
结论:
- BSA-Au复合体的红色光与老化 BSA 的特定阴离子 Au 结合点有关.
- 这与中性Au25纳米团模型的单位核形成相矛盾.
- 在BSA中存在多个特定的Au结合点,影响复杂的光.
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