轴突基因组和关键组织形成调节者的演变
Sergej Nowoshilow1,2,3, Siegfried Schloissnig4, Ji-Feng Fei5
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Nature
|January 25, 2018
概括
墨西哥虫的基因组被测序, 由于反向元素, 发现了大量的内核和跨基因区域. 这种基因组资源有助于研究发育和再生.
科学领域:
- 发育生物学
- 复原医学
- 进化生物学
背景情况:
- 沙兰,特别是墨西哥 (Ambystoma mexicanum),是研究发育,再生和进化的关键模型.
- 墨西哥鱼拥有广泛的分子工具包, 成为分子研究的关键物种.
研究的目的:
- 测序和组装32基基对的墨西哥虫基因组.
- 调查鱼的再生和发育过程的基因组基础.
主要方法:
- 使用长读数测序和光学映射.
- 开发了一种名为MARVEL的新型基因组装器.
- 分析了基因组大小扩张,内/内基因区域和基因含量.
主要成果:
- 已经成功测序并组装了大虫的基因组.
- 主要来自长端重复元件的内部和基因间区域的显著扩张.
- 在发育基因中发现了限制性内核大小的证据,并确定了与肢体再生相关的潜在物种限制基因.
- 轴突基因组缺乏Pax3基因,但Pax7突变是小鼠的Pax3/Pax7缺陷.
结论:
- 阿克索洛特基因组序列是开发和进化研究的宝贵资源.
- 对内部扩张和基因内容的基因分析为再生研究提供了新的途径.
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