对分子和纳米级材料直接核交付的光控制纳米平台的开发
Ya-Xuan Zhu1, Hao-Ran Jia1, Guang-Yu Pan1
1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering , Southeast University , 2 Sipailou Road , Nanjing 210096 , P. R. China.
Journal of the American Chemical Society
|February 7, 2018
概括
研究人员开发了一种新型的纳米平台,使用多面寡合丝素 (POSS),聚乙烯糖醇 (PEG) 和孟加拉 (RB) 来进行光控制的药物和纳米材料的核输送.
科学领域:
- 生物医学工程
- 纳米技术
- 细胞生物学
背景情况:
- 核膜是核向剂的重要障碍,因为核孔的尺寸有限 (9-12纳米).
- 有效的核传递对于包括化疗和纳米医学在内的各种应用至关重要.
研究的目的:
- 为将化学化合物和纳米材料输入细胞核开发一个通用和可控的平台.
- 通过一种可诱导光的机制克服核膜屏障.
主要方法:
- 将含有聚胺的POSS,PEG和孟加拉 (RB) 自组装成纳米粒子 (PPRNP).
- 使用光诱导的单片氧生成来破坏溶酶体并促进核膜透.
- 将化疗剂和纳米材料装入PPRNP进行输送.
主要成果:
- PPR NPs被内化到细胞中,并定位在溶酶体中.
- 轻微的光照射触发了单片氧的产生,破坏了 lysosomes 并导致 PPR NPs 在核膜上积累.
- 通过光控制的化学治疗剂 (10- 胺,dcetaxel),光染料 (DiD) 和纳米材料 (普鲁士蓝色NP,金色纳米棒) 的成功输送.
- 该纳米平台展示了一般适用性,并避免了复杂的连接物修饰.
结论:
- 开发的PPRNP平台提供了高度可控和通用的核交付方法.
- 这种光激活的策略有效地绕过了分子有效载荷和纳米材料的核膜屏障.
- 纳米平台为传统的核输送方法提供了一个有前途的替代方案,增强了治疗和诊断潜力.
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