通过下游寡核酸增强非酶性RNA原始扩展的结构性推理
Wen Zhang1,2, Chun Pong Tam1,3, Lijun Zhou1,2
1Howard Hughes Medical Institute and Center for Computational and Integrative Biology, Massachusetts General Hospital , Boston, Massachusetts 02114, United States.
Journal of the American Chemical Society
|February 8, 2018
概括
一种下游的寡核酸通过稳定激活的单体来增强非酶性RNA复制. 这一发现揭示了前生物的RNA复制机制以及辅助性寡核酸在早期RNA进化中的作用.
科学领域:
- 生命研究的起源
- 核糖核酸生物化学
背景情况:
- 非酶性RNA原始延伸是前生物RNA复制的一个关键模型.
- 活性 mononucleotides 和 imidazolium-bridged dinucleotides 增加了原料延伸率.
- 下游的寡核酸进一步提高了原料延伸效率.
研究的目的:
- 阐明下游寡核酸增强非酶性RNA原料扩展的机制.
- 通过结晶学研究来研究增强板延伸的结构基础.
- 了解助手寡核酸在前生物RNA合成中的作用.
主要方法:
- 对RNA原型复合物的晶体分析.
- 使用一种不可水解的基质模拟物 - - 瓜诺辛5' - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 酸 (ICG).
- 结合ICG和下游寡核酸的复合物的结构确定.
主要成果:
- 下游的寡核酸,无论其激活状态如何,都会使结合的单体变硬.
- 氧核酸定位激活的单体,以便由3'-基基基素进行有效的攻击.
- 当辅助性寡核酸存在时,初始扩展的预组织得到改善.
结论:
- 下游辅助寡核酸在非酶的原料延伸中起着催化作用.
- 这些寡核酸有利于原料-模板-中间体复合物的反应性构造.
- 这些发现提供了早期RNA复制的合作机制的见解.
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