在可选化和自热体中的原始和可逆的TCA循环
Takuro Nunoura1, Yoshito Chikaraishi2,3,4, Rikihisa Izaki5
1Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. takuron@jamstec.go.jp.
概括
这项研究揭示了Thermosulfidibacter takaii的可逆三酸 (TCA) 循环. 这种古老的碳固化途径使用酸盐合成酶,挑战了以前的TCA循环模型.
科学领域:
- 微生物学
- 生物化学
- 天体生物学
背景情况:
- 降解三酸 (rTCA) 循环是地球生命的基本古代碳固定途径.
- 了解碳固化的进化和机制是理解早期生命和潜在的外星生命的关键.
研究的目的:
- 调查深度分支的化学菌中的碳固定路径.
- 阐明三碳酸 (TCA) 循环的机制和进化意义.
主要方法:
- 基因组分析以确定代谢基因.
- 酶测试以确定酶的功能和调节.
- 在不同的生长条件下分析代谢中间体的代谢概况.
主要成果:
- 在*Thermosulfidibacter takaii*中发现了以前未知的可逆TCA循环.
- 证明TCA循环的方向是由可用的碳源控制的.
- 鉴定酸盐合成酶 (CS) 作为在化学自营条件下驱动rTCA循环的关键酶,绕过依赖ATP的酸盐裂变.
结论:
- 在*Thermosulfidibacter takaii*中,rTCA循环通过酸酶逆行,与传统模型不同.
- 这种可逆的CS机制可能是rTCA循环的原始形式.
- 这些发现表明生命的起源可能是随意的.
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