在内源基因中对piRNA向规则和对piRNA沉默的抵抗
Donglei Zhang1,2, Shikui Tu3,4, Michael Stubna1
1Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
Science (New York, N.Y.)
|February 9, 2018
概括
在C. elegans中,Piwi相互作用RNAs (piRNAs) 可以针对一些不匹配的核酸,解释转基因沉默. 周期性An/Tn集群 (PATC) 对这种沉声机制产生阻力.
科学领域:
- 分子生物学
- 遗传学
- 核糖核酸生物学
背景情况:
- 通过使动物中的可转移元素保持沉默,与piwi相互作用的RNA (piRNA) 对于保持基因组完整性至关重要.
- 没有针对转子子的piRNAs的精确功能在很大程度上仍未被描述.
研究的目的:
- 阐明控制*Caenorhabditis elegans*中的piRNA相互作用的向规则.
- 了解由piRNAs抑制转基因和内源基因的基因机制.
- 识别对piRNA介导的沉默产生抵抗的因素.
主要方法:
- 对*C. elegans*的piRNA向特异性的分析,评估对不匹配的耐受性.
- 设计转基因以逃避piRNA识别并评估它们的表达稳定性.
- 在内源性生殖基因中对piRNA向部位的生物信息预测和分析.
- 调查周期性An/Tn集群 (PATC) 作为潜在的阻力信号.
主要成果:
- 在*C. elegans*中,PiRNA向性容忍有限的不匹配,但在种子区域更喜欢完美配对.
- piRNAs的广泛向能力解释了转基因的观察到的生殖线沉默.
- 设计以避免piRNA识别的转基因表现稳定.
- 许多内源性生殖基因具有预测的piRNA结合部位,而PATC则作为对piRNA沉默的内在盾牌.
结论:
- 这项研究定义了piRNA向的规则,揭示了细微的识别机制.
- 通过piRNA相互作用,C. elegans采用一种独特的策略来区分自我与非自我核酸.
- 了解piRNA向提供了对基因组防御和基因调节的见解.
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