染色体转移使细菌前膨胀并影响细胞形态
Javier Lopez-Garrido1, Nikola Ojkic2, Kanika Khanna1
1Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Cell
|February 10, 2018
概括
在分泌过程中细菌细胞的形状变化是由膜合成和染色体运动驱动的,而不是细胞壁的生长. 膨胀的细菌核体膨胀了体,决定了它的最终卵状形状.
科学领域:
- 微生物学
- 细胞生物学
- 生物物理
背景情况:
- 细菌细胞形状的确定是复杂的,并未完全理解.
- 细胞形态源于细胞组件的物理化学性质.
- 在细菌分泌过程中发生编程的细胞形状变化.
研究的目的:
- 调查细菌细胞在Bacillus subtilis分泌过程中改变形状的机制.
- 确定各种细胞组件和过程对形态发生的贡献.
主要方法:
- 遗传学
- 时间缩影显微镜
- 低温电子断层扫描
- 数学模型
主要成果:
- 前生长取决于膜合成和SpoIIIE介导的染色体转位.
- 糖和蛋白质合成不需要用于胞生长.
- 化核体的胀会导致核体的膨胀和重塑,从而导致核体的移位.
结论:
- 细胞组件之间的生物物理相互作用决定了细菌细胞形态.
- 核状胀是细菌子形成和细胞形状的关键机制.
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