主要神经和外围组织的灵长类动物日间转录组图谱
Ludovic S Mure1, Hiep D Le1, Giorgia Benegiamo1
1Regulatory Biology Laboratory, Salk Institute for Biological Studies, 10010, North Torrey Pines Road, La Jolla, CA 92037, USA.
概括
这项研究绘制了64种组织中的日间基因表达, 这些日常节律提供了特定的组织功能,并且在白天和夜间动物之间存在差异.
科学领域:
- 基因组学
- 时间生物学
- 哺乳动物生理学
背景情况:
- 日常基因表达驱动每日组织功能, 但地图书是有限的, 特别是从非夜生活物种.
- 了解灵长类动物的昼夜节律对于比较生物学和人类健康至关重要.
研究的目的:
- 为 (Papio anubis) 的64个组织创建一个全面的日间转录组图谱.
- 研究基因的组织特异性节奏表达,包括那些涉及至关重要的细胞功能.
- 为了比较白天灵长类动物和夜间动物之间的基因表达的时间组织.
主要方法:
- 在24小时内每隔2小时抽取64个组织的高通量RNA测序.
- 生物信息分析以确定节奏基因表达模式及其相位分布.
- 日间和夜间基因表达时间组织的比较分析.
主要成果:
- 从Papio anubis生成了64个组织的日间转录组图谱,其中包括22个大脑区域.
- 发现高达81.7%的蛋白质编码基因表现出每日的节奏表达.
- 在中央和外围组织中识别了无处不在的基因和独特的时间组织的特定组织节奏表达.
- 在黎明和黄昏期间观察到基因表达的峰值,在夜晚早些时候有休息期.
结论:
- 的转录组表现出广泛的日常节奏,为组织增加了一层功能专业化.
- 白天灵长类动物的日间基因表达模式与夜间动物的基因表达模式有很大不同.
- 这本地图书为了解灵长类生物过程的时间调节提供了宝贵的资源.
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