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通过CRISPR/Cas9的动力机制揭示的功能洞察

Austin T Raper1, Anthony A Stephenson1, Zucai Suo1

  • 1Department of Chemistry and Biochemistry, ‡Ohio State Biochemistry Program, and §The James Comprehensive Cancer Center, The Ohio State University , Columbus, Ohio 43210, United States.

Journal of the American Chemical Society
|February 15, 2018
PubMed
概括

这项研究定义了Streptococcus pyogenes Cas9的动力机制,揭示了缓慢的DNA产物释放使其成为单次转换酶. 了解Cas9的动力学对于基因编辑技术的发展至关重要.

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