组织硬化通过在体内触发集体细胞迁移来协调形态发生
Elias H Barriga1,2, Kristian Franze3, Guillaume Charras1,2
1Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Nature
|February 15, 2018
概括
在胚胎发育过程中,组织硬化引导了细胞的集体迁移. 由胃化运动驱动的中皮硬化,通过机械感知触发神经细胞迁移,将机械与形态发生联系起来.
科学领域:
- 发育生物学
- 细胞力学
- 组织工程
背景情况:
- 集体细胞迁移对于发育和疾病至关重要,涉及分子和机械线索.
- 组织力学对体内集体细胞迁移的影响在很大程度上仍未被探索.
- 虫神经细胞作为集体细胞迁移的模型,类似于癌症入侵.
研究的目的:
- 研究机械线索在Xenopus laevis神经细胞的集体迁移中的作用.
- 阐明组织力学影响胚胎形态的机制.
- 建立胃流动,组织机制和神经细胞迁移之间的联系.
主要方法:
- 对Xenopus laevis胚胎进行机械和分子操作.
- 神经细胞迁移和上皮转化为介质细胞的分析.
- 研究涉及整合素-素-素的机械感知途径.
主要成果:
- 头部中皮质的硬会引发脑神经细胞的集体迁移.
- 神经细胞使用整合素-素-氨酸介导的机械感应来检测中皮硬度.
- 胃化过程中的收延伸增加了中皮硬度,从而协调神经迁移.
结论:
- 中皮硬化既必要又足以启动神经的集体迁移.
- 组织机制,特别是基质刚性,可以在体内触发集体细胞迁移和上皮细胞转变为介质细胞.
- 收延伸作为形态发生的机械协调者,通过改变的组织机制将化与神经迁移联系起来.
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