超明亮和化探针用于细胞和组织中脂质滴的多色成像和跟踪
Mayeul Collot1, Tkhe Kyong Fam1, Pichandi Ashokkumar1
1Laboratoire de Biophotonique et Pharmacologie, CNRS UMR 7213 , Université de Strasbourg, Faculté de Pharmacie , 74, Route du Rhin , 67401 Illkirch , France.
Journal of the American Chemical Society
|February 16, 2018
概括
新的StatoMerocyanines (SMCy) 是明亮的,光稳定的光体,专门标记活细胞和组织中的脂质滴. 这些染料可实现多色成像和LD动态的跟踪,比现有的脂质滴标有优势.
科学领域:
- 细胞生物学
- 有机化学
- 生物物理
背景情况:
- 脂质滴 (LDs) 是参与脂质新陈代谢的关键器官,并与肥胖,糖尿病和癌症等疾病有关.
- 现有的脂质滴标记物,如尼罗河红色,在多色成像和光稳定性方面存在局限性.
- 需要新的光探测器来研究LD.
研究的目的:
- 开发和描述一种新型的化色素染料,即StatoMerocyanines (SMCy),用于脂滴成像.
- 在光特性,特异性和与活细胞和组织成像相容性方面评估SMCy染料的性能.
- 证明SMCy染料在先进的成像应用中具有实用性,包括多色和双光子显微镜.
主要方法:
- 与二氧化碳酸盐衍生物 (SMCy) 结合的印林基梅洛酸的合成.
- SMCy染料的光谱表征,包括吸收,排放,量子产量和分子灭绝系数.
- 在活细胞和组织中染色脂质滴,然后进行光显微镜 (共聚焦,3D,双光子).
- 用于脂滴成像的SMCy染料与尼罗河红色的比较.
主要成果:
- 与水性介质相比,SMCy染料具有从黄色到近红外 (NIR) 的明亮光,在油中显著增强.
- 这些染料具有高分子灭绝系数和量子产量,确保强烈的信号检测.
- 在微小的背景噪音下,SMCy 特别染色活细胞中的脂质滴.
- SMCy染料与细胞固定相容,并可在细胞和组织中进行高质量的3D成像.
- 它们的狭窄的光谱带有助于多色成像,而高二光子吸收的截面使其能够进行先进的组织成像.
- SMCy染料可以追踪细胞之间的脂质滴交换,证明细胞间的通信.
结论:
- 斯塔托米洛 (SMCy) 是一种新型的高效和特定的光探针,用于脂质滴研究.
- SMCy染料克服了现有标记物的局限性,使得LD的先进多色和深层组织成像成为可能.
- 这些光体是研究脂质滴在生理和疾病中的作用的宝贵工具,包括细胞间脂质转移.
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