脂质体辅助代谢甘氨酸标记的机制研究和多重化
Yuting Sun, Senlian Hong, Ran Xie
1Departments of Molecular Medicine and Immunology and Microbiology , The Scripps Research Institute , La Jolla , California 92037 , United States.
Journal of the American Chemical Society
|February 16, 2018
概括
脂质体辅助生物对等报告器 (LABOR) 策略使细胞类型选择性代谢甘氨酸标记成为可能. 这种方法利用内细胞解剖救助途径进行精确的甘氨酸成像和分析.
科学领域:
- 生物化学
- 细胞生物学
- 化学生物学
背景情况:
- 代谢甘氨酸标记对于甘氨酸成像和甘氨酸蛋白质分析至关重要.
- 一个主要的局限性是当前方法缺乏细胞类型的选择性.
- 脂质体辅助生物直角报告器 (LABOR) 策略提供了一个潜在的解决方案.
研究的目的:
- 阐明劳动战略的基本机制.
- 探索 LABOR 选择性标签的多重复合能力.
- 扩大LABOR针对不同细胞类型的适用性.
主要方法:
- 通过受体介导的内细胞分裂来研究LABOR的机制.
- 从脂质体中释放出 lysosomal 贩运和 azidosugar.
- 针对基糖标签的各种联体受体对的评估.
主要成果:
- LABOR通过一个内细胞复合救援机制运作,在溶酶体中释放亚糖.
- 亚糖通过 lysosomal 糖载体运输到细胞质中.
- 在共同培养的细胞中使用不同的化学报告剂成功进行多重标记.
结论:
- 阐明的机制支持LABOR的细胞类型选择性.
- LABOR是多功能性的,可以容纳各种类型的连接体,以提供有针对性的输送.
- 多重复合的LABOR可以在不同的细胞群中同时选择性地标记甘氨酸.
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