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相关概念视频

Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

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相关实验视频

Updated: Jun 1, 2026

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
09:30

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Published on: July 13, 2017

兰巴抑制器和兰巴克罗如何区分OR1和OR3

A Hochschild, J Douhan, M Ptashne

    Cell
    |December 5, 1986
    PubMed
    概括
    此摘要是机器生成的。

    菌体兰巴抑制剂和Cro蛋白区分DNA运算子序列. 蛋白质中的特定氨基酸和操作者中的特定基对决定了这些识别偏好.

    更多相关视频

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
    08:56

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

    Published on: July 17, 2018

    相关实验视频

    Last Updated: Jun 1, 2026

    Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
    09:30

    Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

    Published on: July 13, 2017

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
    08:56

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

    Published on: July 17, 2018

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 蛋白质-DNA相互作用

    背景情况:

    • 兰巴抑制剂和兰巴克罗蛋白与菌素染色体上共享的操作位点结合.
    • 这些蛋白质对特定的操作器序列 (OR1和OR3) 具有明显的结合偏好.

    研究的目的:

    • 调查兰巴抑制剂和Cro蛋白的差异性结合特性的分子基础.
    • 识别关键的氨基酸残留物和负责区分运算符序列的DNA基对.

    主要方法:

    • 使用局部定向突变发生来改变运算子序列内的基对.
    • 氨基酸替代物被引入了抑制剂和Cro蛋白的识别螺旋和氨基终端臂.
    • 分析了结合亲和关系,以确定这些修改对蛋白质-DNA相互作用的影响.

    主要成果:

    • 蛋白质偏好是由识别螺旋和氨基末端臂 (用于抑制器) 的残留物5和6决定的.
    • 区分的关键DNA操作员位置是位置3 (对于Cro) 和位置5和8 (对于抑制器).
    • 特定的氨基酸-蛋白质和基对相互作用是抑制剂和Cro的独特识别能力的基础.

    结论:

    • 该研究阐明了兰巴抑制剂和Cro实现序列特定DNA结合的分子机制.
    • 这些发现有助于更深入地了解相关蛋白质如何演变出不同的DNA识别配置文件.
    • 已识别的残留物和基对是操作员歧视的关键决定因素.