概括
研究人员确定了一种70kd的蛋白质,该蛋白质与前mRNA中的3'拼接部位结合. 这种蛋白与小核核核糖核蛋白颗粒结合,对于mRNA拼接至关重要.
科学领域:
- 分子生物学分子生物学
- 在RNA分离过程中.
- 蛋白质生物化学 蛋白质生物化学
背景情况:
- 预mRNA剪接是基因表达的一个基本过程.
- 特定的蛋白质因子对于识别前mRNA上的拼接部位至关重要.
- 3'结合部位是结合体组合的关键识别元素.
研究的目的:
- 确定和描述负责在HeLa细胞核提取物中识别3'拼接位的蛋白质成分.
- 为了研究这种3'拼接位结合因子的结合特性和相互作用.
主要方法:
- 希拉细胞核提取物的分离.
- 检测RNAase T1保护试验以检测RNA结合活性.
- 使用特定抗体 (抗Sm,抗-2,2,7-trimethylguanosine) 进行蛋白质涂抹和西部涂抹分析.
- 生物化学测试以评估蛋白质-RNA相互作用和Mg++度依赖性.
主要成果:
- 由抗Sm抗体识别的70 kd蛋白被发现与3'拼接位结合活性共净化.
- 70kd的多结合了前mRNA,偏好在3'拼接接口附近的序列.
- 这种结合成分在低Mg++条件下与小核核核糖核蛋白颗粒 (snRNP) 相结合.
结论:
- 含有70kd Sm的蛋白质是在mRNA前拼接过程中识别3'拼接部位的关键因素.
- 这种蛋白质与snRNP的关联受Mg++度的调节,影响结合体组合.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...


