Piezo1通道的结构和机制
Qiancheng Zhao1,2,3, Heng Zhou1,2, Shaopeng Chi1,2,3
1School of Pharmaceutical Sciences or Life Sciences, Tsinghua University, Beijing 100084, China.
Nature
|February 23, 2018
概括
研究人员揭示了Piezo1通道的结构, 这一发现揭示了它们独特的螺旋形状和杆式机械传导机制,
科学领域:
- 生物物理
- 分子生物学
- 细胞生物学
背景情况:
- 机械敏感的皮埃佐通道对于细胞的机械感应至关重要.
- 皮耶索道的详细结构和封闭机制仍然难以捉摸.
研究的目的:
- 确定鼠标Piezo1通道的高分辨率结构.
- 阐明Piezo1介导机械传导的分子组成部分和机制.
主要方法:
- 使用电子冷显微镜 (cryo-EM) 来确定小鼠Piezo1的结构.
- 进行了功能性测试,以调查已识别的结构成分在机械传导中的作用.
主要成果:
- 这项研究确定了小鼠Piezo1的三刀式冷EM结构.
- 九个重复的跨膜螺旋单元 (THU) 组装成曲的叶片,最后的螺旋形成孔隙.
- 一个中心的细胞内束状结构促进了杆状的运动,将THU连接到孔隙,并使机械关成为可能.
结论:
- Piezo1具有独特的38-跨膜螺旋拓,具有明显的机械传导元素.
- 一个类似杆的机制,涉及细胞内光束和THU,控制Piezo1对机械刺激的反应.
- 细胞外循环或细胞内光束的破坏会损害Piezo1的机械激活,
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