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相关概念视频

Implementation of a Real-Time Psychosis Risk Detection and Alerting System Based on Electronic Health Records using CogStack07:31

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We demonstrate how to deploy a real-time psychosis risk calculation and alerting system based on CogStack, an information retrieval and extraction platform for electronic health...
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This article presents a protocol based on Hydra — a web-based system for clinical decision support that integrates a full and detailed set of functionalities and services required by physicians for complete cardiovascular analysis, risk assessment, early diagnosis, treatment, and monitoring over...
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This manuscript describes the Research Prioritization by Affected Communities (RPAC) protocol and findings from its use with women at risk for preterm birth. Using the protocol, women identified and prioritized their unanswered questions about pregnancy, birth and neonatal care aimed at influencing research priority setting by funders and...
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There is a critical need for tools and methodologies capable of managing aquatic systems in the face of uncertain future conditions. We provide methods for conducting a targeted watershed assessment that enables resource managers to produce landscape-based cumulative effects models for use within a scenario analysis management...
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The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.
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In this candid interview, Anthony A. James explains how mosquito genetics can be exploited to control malaria and dengue transmission. Population replacement strategy, the idea that transgenic mosquitoes can be released into the wild to control disease transmission, is introduced as well as the concept of genetic drive and the design criterion for an effective genetic drive system. The ethical considerations of releasing genetically-modified organisms into the wild are also...
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相关实验视频

Updated: Jan 19, 2026

Implementation of a Real-Time Psychosis Risk Detection and Alerting System Based on Electronic Health Records using CogStack
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通过Microwell-Seq绘制鼠标细胞地图

Xiaoping Han1, Renying Wang1, Yincong Zhou2

  • 1Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China; Stem Cell Institute, Zhejiang University, Hangzhou 310058, China.

Cell
|February 24, 2018
PubMed
概括

我们开发了Microwell-seq,一个成本高效的单细胞RNA测序平台 (scRNA-seq),用于创建小鼠细胞图谱 (MCA). 这种资源绘制了超过40万个细胞,揭示了新的细胞层次,并使细胞类型的精确定义成为可能.

关键词:
微小的时间细胞类型分类细胞异质性跨组织细胞网络哺乳动物细胞地图鼠标细胞地图scMCA分析一个单细胞RNA-seq单细胞分析转录组分析

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相关实验视频

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科学领域:

  • 基因组学
  • 细胞生物学
  • 生物信息学

背景情况:

  • 单细胞RNA测序 (scRNA-seq) 正在彻底改变细胞类型的分类.
  • 对于复杂的哺乳动物系统,一个全面的单细胞地图仍然难以捉摸.
  • 现有的scRNA-seq方法可能昂贵且难以获得.

研究的目的:

  • 开发一个高吞吐量,低成本的scRNA-seq平台 (Microwell-seq).
  • 构建一个覆盖主要器官的基础小鼠细胞图谱 (MCA).
  • 使用数字基因表达数据建立细胞类型识别的计算管道.

主要方法:

  • 使用简单,便宜的设备开发Microwell-seq平台.
  • 分析了来自不同小鼠组织的40多万个单细胞.
  • 创建一个基于网络的单细胞数字表达数据分析管道.

主要成果:

  • 成功生成小鼠的大规模单细胞数据集.
  • 在以前未被描述的组织中识别新的单细胞层次.
  • 验证Microwell-seq平台的效率和成本效益.
  • 基于数字表达的精确细胞类型定义管道的开发.

结论:

  • 微-seq为scRNA-seq提供了一个可扩展和负担得起的方法.
  • 小鼠细胞地图 (MCA) 为了解哺乳动物细胞多样性提供了宝贵的资源.
  • 开发的分析管道可以从scRNA-seq数据中精确地分类细胞类型.