转录异形转换导致的普遍,协调的蛋白质水平变化
Ze Cheng1, George Maxwell Otto1, Emily Nicole Powers1
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell
|February 24, 2018
概括
基因表达的调节比以前认为的要复杂得多. 一项关于起芽酵母的研究揭示了一种新奇的机制, 转录类型, 不仅仅是丰富, 在发育过程中控制蛋白质水平.
科学领域:
- 分子生物学
- 遗传学
- 发育生物学
背景情况:
- 了解基因调节机制对于破译发育过程至关重要.
- 现有的模型主要关注mRNA水平来预测蛋白质的产生.
研究的目的:
- 在发芽的酵母中研究介质分化的全基因组基因调节机制.
- 同时测量mRNA,翻译和蛋白质水平.
主要方法:
- 同时测量全基因组的mRNA,翻译和蛋白质.
- 在芽酵母中介质分化的分析.
- 对抗相关的mRNA和蛋白质水平的鉴定.
主要成果:
- 数百个mRNA与它们的蛋白质产物呈现出反相关性.
- 超过8%的测量基因 (至少380个) 使用一种新的调节机制.
- 这种机制涉及可翻译和不可翻译的mRNA异型之间的切换.
- 一个转录因子可以协调蛋白质合成的激活和抑制.
结论:
- 发育过程中的基因调节涉及通过转录异形切换调节蛋白质水平的普遍机制.
- 产生的转录类型,而不仅仅是mRNA诱导,决定了蛋白质合成.
- 这一发现为转录后基因调节提供了新的视角.
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