在90天内制造10个分子的压力测试:对工程生物学方法的外部评估
Arturo Casini1,2, Fang-Yuan Chang1,3, Raissa Eluere1,2
1The Foundry , 75 Ames Street , Cambridge , Massachusetts 02142 , United States.
Journal of the American Chemical Society
|February 27, 2018
概括
生物发明公司通过工程生物来推进合成生物学. 一个为期3个月的"压力测试"证明了这种能力,成功地生产了10个目标分子中的6个,并推进了其他分子.
科学领域:
- 合成生物学
- 基因工程
- 生物造发展
背景情况:
- 在医学,农业,工业和国防方面设计有机体的潜力.
- 基因工程的快速技术进步需要能力评估.
研究的目的:
- 通过定时性能测试来评估生物造厂的能力和发现缺陷.
- 在3个月内设计10种预先定义的新分子.
主要方法:
- 一个为期3个月的"压力测试",涉及到设计和构建10个向分子的生物.
- 应用各种基因工程和合成生物学方法.
- 构建DNA,微生物菌株和无细胞系统,加上内部测试开发.
主要成果:
- 成功生成了10个目标分子中的6个或接近的类型.
- 增加特定化合物的标位 (1- 六甲醇,皮罗利特林,帕奇达米辛D).
- 开发了新的生物合成路径,无细胞系统,并编码了广泛的DNA路径.
结论:
- 在生产点分子和推进新生物合成途径方面表现出显著的能力.
- 特定的挑战包括毒性和分析限制.
- 这项研究提供了生物造业绩的基准,并强调了未来发展的领域.
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