![Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F61682.jpg&w=3840&q=50)
智能SERS热点:单个分子可以使用宿主-客户化学定位在等离子纳米连接中
Nam Hoon Kim1, Wooseup Hwang1, Kangkyun Baek1
1Center for Self-assembly and Complexity, Institute for Basic Science , Pohang 37673 , Republic of Korea.
Journal of the American Chemical Society
|February 28, 2018
概括
研究人员开发了一种"智能"热点,用于单分子表面增强拉曼光谱 (SERS) 使用库库比特[6]uril. 这种方法可靠地定位单个分子进行分析,克服了SERS应用中的先前限制.
科学领域:
- 化学物理
- 光谱学
- 纳米技术
背景情况:
- 单分子表面增强拉曼光谱 (SERS) 可以研究复杂的化学和生物过程.
- 在SERS的一个关键挑战是可靠地将单个分子定位在"热点"内进行分析.
研究的目的:
- 开发一种新的策略,用于在等离子纳米连接处的SERS热点内定位和保护单个目标分析物.
- 通过使用高亲和度分片来研究对热点亲和度低的分子.
主要方法:
- 使用醇功能化黄素[6] (CB[6]) 作为银纳米粒子和金属基质之间的分子间隔器生成"智能"热点.
- 将目标分析物与精氨酸等分子结合,以增强对CB的亲和力[6].
- 使用双分析实验和不同的连接器长度来测量取决于距离的SERS强度.
主要成果:
- "智能"热点可靠地容纳只有少数,通常是单一的分析分子.
- 可复制基质处理导致96%的热点含有单个分析分子.
- 随着热点中心距离的平方,SERS强度下降,单分子SERS截面随着银纳米粒子直径的增加.
结论:
- 开发的"智能"热点战略有效地解决了单分子本地化在SERS中的挑战.
- 这种方法提高了SERS分析单个分子的能力,包括具有低内在亲和力的分子.
- 这些发现为化学和生物学中先进的单分子SERS应用提供了基础.
更多相关视频
10:43Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
Published on: July 21, 2023
4.1K
06:34In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
Published on: September 2, 2016
6.8K
相关概念视频
01:45Positive Regulator Molecules
136.5K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.5K
02:39Positive Regulator Molecules
6.9K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.9K
03:25Chemistry of Carbohydrates
91.1K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
91.1K
03:25Chemistry of Carbohydrates
10.8K
10.8K
02:58Chemistry of the Cell
48.4K
The cell is chemically composed of water, organic molecules and inorganic ions.
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
48.4K
02:58Chemistry of the Cell
9.6K
9.6K